Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Design and evolution of the tetracycline repressor into sulfonylurea herbicide-responsive gene switches for field crops.

Nature communications·2026
Same author

Neuropixels Opto: combining high-resolution electrophysiology and optogenetics.

Nature methods·2026
Same author

Topographic structure and function of locus coeruleus norepinephrine neurons.

bioRxiv : the preprint server for biology·2026
Same author

Functional reorganization of motor cortex connectivity during learning.

bioRxiv : the preprint server for biology·2026
Same author

iGABASnFR2 is an improved genetically encoded protein sensor of GABA.

eLife·2026
Same author

Sardine: A modular framework for developing data acquisition and near real-time analysis applications.

PloS one·2026

相关实验视频

Updated: May 6, 2026

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
12:32

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Published on: June 26, 2013

23.7K

超敏感的光蛋白用于成像神经元活动.

Tsai-Wen Chen1, Trevor J Wardill, Yi Sun

  • 1Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA.

Nature
|July 23, 2013
PubMed
概括

研究人员开发了新的超敏感蛋白传感器 (GCaMP6),用于成像神经活动. 这些先进的GCaMP6传感器可以更好地检测各种生物体和大脑区域的神经元信号.

更多相关视频

Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo
12:48

Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo

Published on: May 12, 2016

12.4K
Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
08:59

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Published on: July 16, 2021

2.5K

相关实验视频

Last Updated: May 6, 2026

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
12:32

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Published on: June 26, 2013

23.7K
Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo
12:48

Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo

Published on: May 12, 2016

12.4K
Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
08:59

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Published on: July 16, 2021

2.5K

科学领域:

  • 神经科学是一个神经科学.
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 光传感器是可视化神经活动的关键工具.
  • 现有的传感器在灵敏度和性能上都有局限性.
  • 了解神经电路动态需要高分辨率的成像工具.

研究的目的:

  • 为了开发和描述一种新的超敏感蛋白质传感器家族,GCaMP6.
  • 评估GCaMP6传感器在各种生物系统和神经元区的性能.
  • 用GCaMP6.6研究神经回路的空间和时间动态.

主要方法:

  • 基于结构的蛋白质工程的突变发生.
  • 基于神经元的查用于功能评估.
  • 在模型生物 (斑马鱼,,小鼠) 和培养的神经元中的体内和体外成像.
  • 电生理学和成像在特定的皮质层和神经元类型.

主要成果:

  • 开发了GCaMP6,一种超敏感蛋白质传感器家族,性能优于现有的传感器.
  • 在小鼠视觉皮层中,GCaMP6可靠地检测到单动作潜力和突触瞬态.
  • 在几周的时间里,在树突棘中证明了定向调整的稳定性.
  • 在GABAergic神经元的树突段中发现了定向调整,但在它们的体质中没有观察到.

结论:

  • GCaMP6传感器为成像神经活动提供了前所未有的灵敏度.
  • 这些传感器可以在多个尺度上详细调查神经电路组织和动态.
  • GCaMP6促进了对突触功能,神经元编码和电路可塑性的研究.