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使用离子化学交换和转移19F磁共振成像的金属离子传感

Amnon Bar-Shir¹, Assaf A Gilad, Kannie W Y Chan

¹Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.

Journal of the American Chemical Society

|August 3, 2013

概括

这项研究引入了一种新的方法,用于在深层组织中使用-19 (19F) 磁共振成像检测离子 (Ca2+). 该技术可以放大信号,使得对Ca2+度的敏感,非侵入性成像成为可能.

科学领域:

生物医学成像技术生物医学成像技术
化学生物学化学生物学
核磁共振光谱学核磁共振光谱学

背景情况:

在深层组织中非侵入性检测自由金属离子是具有挑战性的.
金属离子在许多生物过程中起着至关重要的作用.
目前用于深层组织金属离子检测的方法有限.

研究的目的:

开发一种用于深层组织中特定和敏感检测离子 (Ca2+) 的新方法.
将化学交换和转移 (CEST) 与 19F NMR 光谱学结合起来,用于 Ca2+ 成像.
克服现有的非侵入性金属离子检测技术的局限性.

主要方法:

使用1,2-bis(o-aminophenoxy) ethane-N,N,N,N',N'-四酸的5,5'-二衍生物 (5F-BAPTA) 作为一个Ca2+传感器.
在Ca2+束的19F频率上使用射频标记.
检测到标签转移到没有Ca2+的19F频率来放大Ca2+信号.
杆基质结合动力学用于信号放大.

主要成果:

在Ca2+结合时,通过19F的化学转移变化 (Δω) 实现了对Ca2+存在的特定感知.
成功获得了Ca2+的磁共振图像.
通过信号放大,通过高灵敏度证明了低Ca2+度的间接检测.

结论:

开发的基于CEST的19F核磁共振方法能够在深层组织中对Ca2+进行敏感的,非侵入性的成像.
这种方法在监测生物相关金属离子度方面取得了重大进展.
该技术对生物和医学领域的各种诊断和研究应用具有前景.

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