相关实验视频
Updated: May 9, 2026
13:04
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Published on: March 1, 2019
一个长的非编码RNA介导免疫反应基因的激活和抑制
Susan Carpenter1, Daniel Aiello, Maninjay K Atianand
1Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Science (New York, N.Y.)
|August 3, 2013
概括
一种新发现的长非编码RNA (lncRNA),lincRNA-Cox2,在调节免疫系统的炎症反应中起着至关重要的作用. 它控制免疫基因的激活和抑制,作为抗菌防御的关键调节者.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 遗传学是一种遗传学.
背景情况:
- 炎症基因表达对抗微生物防御至关重要.
- 这个过程涉及转录因子,联合调节剂和染色质修饰剂.
- 长非编码RNAs (lncRNAs) 越来越多地被认为是细胞过程的调节者.
研究的目的:
- 识别和描述炎症反应中的新型调节分子.
- 研究 lncRNAs 在免疫基因调节中的作用.
- 阐明lincRNA-Cox2在先天免疫中的功能.
主要方法:
- 通过模式识别受体 (例如,Toll-like受体) 诱导的lncRNAs的识别.
- 在免疫基因表达中对lincRNA-Cox2的功能研究.
- 生物化学分析以确定lincRNA-Cox2.2的蛋白相互作用.
主要成果:
- 一种特定的lncRNA,lincRNA-Cox2,被确定为炎症基因表达的关键调节者.
- lincRNA-Cox2调解不同免疫基因组的激活和抑制.
- 通过lincRNA-Cox2进行的转录抑制涉及与异质核核核核蛋白蛋白A/B和A2/B1.1的相互作用.
结论:
- lincRNA-Cox2是炎症反应回路的关键调节成分.
- 这种lncRNA充当广泛作用的调节剂,影响免疫基因表达的多个方面.
- 这些发现凸显了lncRNAs在先天免疫系统功能中的重要性.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...