在人体细胞中,转录因子结合发生在围绕凝聚性点形成的密集集群中
Jian Yan1, Martin Enge, Thomas Whitington
1Science for Life Laboratory, Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm 14183, Sweden.
Cell
|August 20, 2013
概括
转录因子 (TFs) 在细胞分裂后使用凝聚素作为细胞记忆重新结合DNA位点. 凝聚蛋白固TF集群,确保DNA复制和染色体凝聚后的精确基因调节.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 转录因子 (TFs) 对于基因调节至关重要,但在细胞分裂过程中被移除.
- 在DNA复制和染色体凝聚后,TFs重新定位到它们的特定结合点的机制仍然不太清楚.
研究的目的:
- 研究转录因子如何在细胞分裂后恢复与染色质的结合.
- 阐明凝聚素在转录因子的重新结合中的作用.
主要方法:
- 对人类结直肠癌细胞中转录因子结合模式的分析.
- 研究凝聚素耗尽对DNA可访问性和TF结合性的影响.
- 在细胞周期的S和M阶段观察凝聚素和TF结合动态.
主要成果:
- 转录因子结合在染色质上高度聚集.
- 这些集群主要位于凝聚力周围.
- 凝聚素的损失降低了DNA的可访问性和TF集群的形成.
- 在S阶段,凝聚素仍然与TF集群部位结合,保持姐妹染色体,并在TF驱逐后的M阶段持续存在.
结论:
- 凝聚素作为转录因子集群的关键.
- 凝聚素充当细胞记忆,在DNA复制和染色体凝聚后促进TF结合位的重新建立.
- 这种机制确保了在整个细胞周期中精确的基因调节连续性.
相关概念视频
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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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