类似谷氨酸受体的基因介导着叶子对叶子的伤口信号传递
Seyed A R Mousavi1, Adeline Chauvin, François Pascaud
1Department of Plant Molecular Biology, University of Lausanne, Biophore, CH-1015 Lausanne, Switzerland.
Nature
|August 24, 2013
概括
受伤的植物叶子使用电信号信号损伤,从而触发防御反应. 特定的谷氨酸受体类基因 (GLRs) 对于这种长距离的伤口通信至关重要.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物物理学的生物物理.
背景情况:
- 受伤的叶子通过远距离信号传输损伤信号到未受损的部分.
- 这种信号通路涉及斯酸盐的产生,斯酸盐是植物防御机制的关键调节者.
研究的目的:
- 研究植物中远距离伤口信号的基础生物物理机制.
- 识别涉及伤口信号传输和随后的防御反应的遗传组件.
主要方法:
- 使用非侵入性电极在叶子受伤后绘制Arabidopsis thaliana的表面潜力变化图.
- 测量了jasmonoyl-isoleucine积累,并分析了对当前注射的反应中的转录组变化.
- 选了34个膜蛋白突变系,重点关注GLR基因.
主要成果:
- 在未受损的叶子中,受伤诱导的膜脱极化与莉花的信号域相关.
- 目前的注射刺激了斯莫诺伊尔-异氨酸的积累,并改变了与斯莫纳酸信号相关的基因表达.
- 在GLR3.2,GLR3.3和GLR3.6基因的突变减少了伤口诱导的表面潜能变化,GLR3.3和GLR3.6突变显示在远叶的斯蒙酸响应基因表达减少.
结论:
- 这项研究为了解植物远距离伤口信号提供了基因基础.
- 鉴定了植物GLUTAMATE受体类基因作为器官间伤口通信的关键参与者,类似于动物突触活动基因.
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