相关实验视频
Updated: May 8, 2026
14:17
Nuclear Transfer into Mouse Oocytes
Published on: November 30, 2006
核波1是重新编程卵细胞转录和正常发育所需的
Kei Miyamoto1, Marta Teperek, Kosuke Yusa
1Wellcome Trust/Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, Cambridge, UK. k.miyamoto@gurdon.cam.ac.uk
Science (New York, N.Y.)
|August 31, 2013
概括
克塞诺普斯Wave1是一种核蛋白,对于重编程体细胞DNA和在胚胎发育期间激活基因至关重要. 缺少它会导致发育缺陷,并阻碍基因激活.
科学领域:
- 发育生物学是发展生物学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 卵子和卵细胞对精子和体内核进行重新编程,这对发育至关重要.
- 核活性蛋白和活性蛋白结合蛋白与转录有关,但它们的作用尚不清楚.
研究的目的:
- 研究Xenopus Wave1在转录重编程和胚胎发育中的作用.
主要方法:
- 波1在卵细胞核中的定位.
- 在Xenopus胚胎中的Wave1敲击.
- 对霍克斯基因激活和发育异常的分析.
- 调查Wave1与转录机器的结合.
主要成果:
- Xenopus Wave1存在于卵细胞核中,对于转录重编程至关重要.
- 波1敲击导致异常发育和受损的霍克斯基因激活.
- 核波1与活性转录因子和RNA聚合酶II相互作用.
结论:
- 波1被确定为一种母性重编程因子.
- 波1在发育过程中对基因激活起着至关重要的作用.
相关概念视频
01:24Methods of Nuclear Reprogramming
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
01:14Introduction to Nuclear Reprogramming
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
01:29Somatic to iPS Cell Reprogramming
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
02:54Nucleosome Remodeling
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...