对RNP构成的全转录组图谱揭示了各种类型的mRNA和lncRNAs
Alex Charles Tuck1, David Tollervey1
1The University of Edinburgh, Wellcome Trust Centre for Cell Biology, Michael Swann Building, Kings Buildings, Edinburgh EH9 3JR, UK.
Cell
|September 3, 2013
概括
使者RNAs (mRNAs) 和长非编码RNAs (lncRNAs) 分享处理步骤,但在命运上有所不同. 转录命运是由3'末端形成决定的,大多数 lncRNA 接受核监测.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- 细胞基因组产生不同的信使RNA (mRNA) 和长非编码RNA (lncRNA).
- 这两种RNA类型都由RNA聚合酶II转录,并经历5'封闭和3'多化.
- 一个关键的区别在于mRNA转化为蛋白质,而lncRNAs不编码蛋白质.
研究的目的:
- 研究区分mRNA和lncRNA命运的分子机制.
- 为了确定转录组范围内的关键RNA处理,出口和酵母酵母的周转因素的目标.
- 了解转录成熟路径在mRNAs和lncRNAs之间如何分离.
主要方法:
- 在芽酵母中对RNA处理因子的全转录组分析.
- 对mRNA和lncRNA成熟路径的比较分析.
- 确定影响3'终端形成和核出口的因素.
主要成果:
- 转录命运主要在3'端处理过程中确定.
- 大多数lncRNA都被指向核RNA监控通路.
- 一个具有mRNA样3'末端的lncRNAs子集被输出到细胞质中.
- Hrp1和Nab2蛋白在mRNA处理和lncRNA监测中起着双重作用.
- 一个mRNAs的子集表现出"lncRNA-like"特征,并通过核监控进行调节.
结论:
- 3'末端形成是mRNA与lncRNA命运决定的关键检查点.
- 像Hrp1和Nab2这样的RNA处理因子编排转录命运决定.
- 核监测机制也会影响某些mRNA的成熟和出口.
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