在体内进行重新编程,产生具有全能性特征的瘤和iPS细胞
María Abad1, Lluc Mosteiro, Cristina Pantoja
1Tumour Suppression Group, Spanish National Cancer Research Centre (CNIO), Madrid E-28029, Spain.
Nature
|September 13, 2013
概括
诱导多能干细胞 (iPS细胞) 可以在生物体内产生 (体内重编程). 这些细胞表现出全能特征,为再生医学提供了新的途径.
科学领域:
- 干细胞生物学 干细胞生物学
- 再生医学是一种再生医学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 诱导多能干细胞 (iPS细胞) 提供治疗潜力.
- 在组织内进行体内重编程的可行性在很大程度上仍未被探索.
研究的目的:
- 调查小鼠体内生物重编程的可能性和特征.
- 为了确定重新编程因素是否可以诱导活体组织中的多能性.
主要方法:
- 在小鼠中暂时诱导Oct4,Sox2,Klf4和c-Myc.
- 在各种器官中分析瘤形成和细胞标记物 (NANOG).
- 骨髓移植以评估血液细胞的细胞重编程.
- 活体生成的iPS细胞的转录组分析与体外iPS细胞和胚胎干细胞 (ES细胞) 相比.
主要成果:
- 在因子诱导后,瘤在多个器官中形成,表明成功的体内重编程.
- 在胃,肠,胰腺和脏中发现了表达NANOG的脱差细胞.
- 造血细胞在体内被重新编程,血液中检测到循环的iPS细胞.
- 在体内,iPS细胞与ES细胞的相似性更大,对 trofhectoderm 血统的贡献更大,这表明它处于一个更原始的状态.
结论:
- 在体内重新编程是可行的,可以产生具有全能特性的细胞.
- 与标准的iPS或ES细胞相比,体内生成的iPS细胞具有独特的特性.
- 这些发现对未来的再生医学应用有重大影响.
相关概念视频
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