长非编码RNAs在增强剂生物学中开启了一个新时代
Ulf Andersson Ørom1, Ramin Shiekhattar
1Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany.
Cell
|September 17, 2013
概括
增强剂相关的长非编码RNAs (lncRNAs) 从远处激活基因转录. 这项研究探讨了lncRNA在增强功能,蛋白相互作用和染色质结构中的作用.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 长非编码RNAs (lncRNAs) 是基因表达的关键调节者.
- 增强剂是控制转录的调节性DNA元素.
- 正在研究 lncRNAs 影响增强剂活性的精确机制.
研究的目的:
- 审查了解ncRNA参与增强器功能的近期进展.
- 阐明 lncRNA 表达在转录激活中的作用.
- 探索 lncRNAs 如何与蛋白质因子结合并调节色素结构.
主要方法:
- 关于 lncRNAs 和增强剂的当前研究的文献综述.
- 对研究蛋白质-lncRNA相互作用的研究进行分析.
- 对 lncRNA介导的染色质重塑的数据的检查.
主要成果:
- lncRNAs可以在很远的距离,甚至跨染色体,以调节目标基因转录.
- 特定的lncRNAs被招募到增强剂中,并促进它们的功能.
- lncRNA与各种蛋白质因子相互作用,调节增强剂-促进剂通信和染色质可访问性.
结论:
- lncRNAs是增强器功能,桥接增强剂和促进剂的关键组成部分.
- 了解lncRNA-蛋白相互作用及其对染色质的影响对于破译基因调节至关重要.
- 在涉及异常基因表达的疾病中,lncRNAs是治疗干预的有希望的目标.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...