Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Prevalence and burden of coronary artery disease in young adults undergoing clinically indicated coronary CT angiography.

Open heart·2026
Same author

A study to measure the utility of an AI-enhanced reporting tool in assisting busy CCTA readers with REPORT generation (SMART-REPORT).

BMC medical imaging·2026
Same author

Charting New Territory: Systematic Evaluation of the Drug Potential of <i>N</i>-Trifluoromethyl Amides, Ureas & Carbamates.

Journal of medicinal chemistry·2026
Same author

Incidental Renal Cell Carcinoma in an Active-Duty Fighter Pilot.

Aerospace medicine and human performance·2026
Same author

Vibecotamab for measurable residual disease in acute myeloid leukemia and for myelodysplastic syndromes and chronic myelomonocytic leukemia after hypomethylating agent failure: a phase II study.

Journal of hematology & oncology·2026
Same author

Investigational clinical trial agents versus conventional second salvage therapies in patients with relapsed/refractory acute myeloid leukemia.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026

相关实验视频

Updated: May 7, 2026

Light-driven Enzymatic Decarboxylation
09:58

Light-driven Enzymatic Decarboxylation

Published on: May 22, 2016

一个高效的光驱动P450 BM3生物催化剂.

Ngoc-Han Tran1, Daniel Nguyen, Sudharsan Dwaraknath

  • 1Department of Chemistry, San José State University , One Washington Square, San José, California 95192-0101, United States.

Journal of the American Chemical Society
|September 18, 2013
PubMed
概括

研究人员创建了混合酶,将P450 BM3血红蛋白域与光敏感剂结合起来. 这一创新使光驱动的P450反应成为可能,绕过了减少酶的需要,并提高了C-H键功能化的催化效率.

更多相关视频

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions
10:21

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions

Published on: October 5, 2019

[(DPEPhos)(bcp)Cu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst
09:12

[(DPEPhos)(bcp)Cu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst

Published on: May 21, 2019

相关实验视频

Last Updated: May 7, 2026

Light-driven Enzymatic Decarboxylation
09:58

Light-driven Enzymatic Decarboxylation

Published on: May 22, 2016

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions
10:21

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions

Published on: October 5, 2019

[(DPEPhos)(bcp)Cu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst
09:12

[(DPEPhos)(bcp)Cu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst

Published on: May 21, 2019

科学领域:

  • 生物化学和生物技术
  • 酶工程是什么? 酶工程是什么?
  • 光催化作用的光催化

背景情况:

  • 细胞染色体P450酶 (P450s) 是C-H键功能化的关键基酸盐酶.
  • 传统的P450催化依赖于减少酶酶进行电子转移,限制了它们的应用范围.
  • 开发减少酶独立的P450系统对于更广泛的生物催化应用是必不可少的.

研究的目的:

  • 设计混合P450 BM3血红蛋白域,能够使用可见光进行催化反应.
  • 为了规避P450介导功能化反应中减少酶的要求.
  • 通过光敏剂集成来增强酶稳定性和催化效率.

主要方法:

  • 通过对P450 BM3血红组域共振连接 (II) 光敏剂来构建混合酶.
  • 可见光辐射驱动催化P450反应,特别是基化.
  • 对光驱催化剂的酶活性,稳定性和循环数的表征.

主要成果:

  • 成功开发出一种高度活性和稳定的混合酶.
  • 这种工程酶有效地催化了酸的光驱动氧化.
  • 取得的总营业额为935个,初始反应速率为125mol产品/...mol酶/分钟).

结论:

  • 混合P450酶包含光敏感剂,为减少酶独立生物催化提供了一个有前途的途径.
  • 可见光辐射可以有效地驱动P450催化C-H功能,扩大催化可能性.
  • 开发的混合酶在选择性氧化反应中显示出工业应用的巨大潜力.