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一个表皮微RNA通过控制细胞糖化状态来调节神经元迁移

Mikael Egebjerg Pedersen¹, Goda Snieckute, Konstantinos Kagias

¹Biotech Research and Innovation Centre, University of Copenhagen, Ole Maaløes Vej 5, Copenhagen, Denmark.

Science (New York, N.Y.)

|September 21, 2013

概括

在Caenorhabditis elegans的微RNA mir-79保持蛋白质甘氨酸的恒常性. 失去mir-79会导致神经发育缺陷,因为它会扰乱肝硫酸盐生物合成和神经元迁移.

科学领域:

发育生物学发展生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

蛋白质甘油的糖化对调节动物发育至关重要.
维持糖链平衡对于适当的细胞功能至关重要.

研究的目的:

为了研究Caenorhabditis elegans微型RNA mir-79在蛋白质甘氨酸平衡中的作用.
确定 mir-79 调节发育路径的目标和机制.

主要方法:

使用Caenorhabditis elegans作为一个模型生物.
研究了microRNA mir-79及其目标基因 (SQV-5,SQV-7) 的功能.
分析了神经发育缺陷和肝素硫酸盐生物合成途径.

主要成果:

微RNA mir-79直接向冠状素合成酶 (SQV-5) 和一个UDP-糖载体 (SQV-7).
失去mir-79会导致皮肤上SQV-5和SQV-7的失调.
这导致肝素硫酸盐生物合成受损,影响LON-2/glypican通路并导致神经发育缺陷,包括神经元迁移受损.

结论:

鉴定了一种保存的微RNA调节轴 (mir-79) 控制蛋白质甘氨酸恒温.
证明mir-79对于维持糖链平衡和C. elegans的适当神经发育至关重要.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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