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一种看似非特异性的RNA结合蛋白中的隐藏特异性

Ulf-Peter Guenther¹, Lindsay E Yandek, Courtney N Niland

¹Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA.

|September 24, 2013

概括

即使是看似不特定的RNA结合蛋白,如C5,在结合位点之间也表现出显著的区别. 这表明,特定和非特定的RNA结合可能代表着亲和分布的连续性.

科学领域:

分子生物学分子生物学
生物化学生物化学
遗传学遗传学是一个

背景情况:

核酸结合蛋白通常根据它们的结合部位特征被分为特异性或非特异性.
特定的蛋白质与定义的序列或结构特征结合,而非特定的蛋白质与缺乏这种特征的部位结合.
对非特异性蛋白质的结合机制仍然不太了解,特别是它们在不同结合位点之间进行区分的能力.

研究的目的:

系统地研究看似非特异性RNA结合蛋白C5.5的基质结合.
要确定C5在不同的结合位变异之间有多大程度的区别.
阐明C5.5对基质认可的规则.

主要方法:

利用高通量测序动力学 (HITS-KIN) 方法,同时分析数千种RNA的处理过程.
测量了C5对其基质结合部位中所有可能的序列变异的功能结合亲和力.
系统地检查了由Escherichia coli的RNase P的蛋白质子单元C5结合基质的结合.

主要成果:

C5在不同的结合位变异之间表现出明显的区别,其亲缘关系以数量级不同.
C5的功能亲和关系的分布类似于高度特定的核酸结合蛋白的分布.
C5将其生理性RNA目标与中等亲和关系结合在一起,而不是最高亲和关系,揭示了隐藏的特异性.

结论:

显然非特异性RNA结合与特异性结合并没有根本的区别,而是代表了共同亲和分布的不同部分.
定义了C5对基质识别的规则,揭示了细胞基质对RNase P.的隐藏特异性.
这些发现挑战了特定和非特定核酸结合蛋白之间的传统二分法.

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