用可开启的光探针对活细胞中的不稳定铁分子成像 (II) 池进行成像
Ho Yu Au-Yeung1, Jefferson Chan, Teera Chantarojsiri
1Departments of Chemistry and ‡Molecular and Cell Biology and §Howard Hughes Medical Institute, University of California , Berkeley, California 94720, United States.
Journal of the American Chemical Society
|September 26, 2013
概括
研究人员开发了Iron Probe 1 (IP1),这是一个新的光工具,用于监测细胞中的性铁. 这种探测器有助于可视化铁含量及其变化,有助于研究铁的调节和相关的细胞应激.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 化学生物学 化学生物学
背景情况:
- 铁对生命至关重要,但其失调会导致细胞损伤.
- 了解不稳定的铁池对于研究铁平衡至关重要.
- 现有的检测铁的方法有局限性.
研究的目的:
- 开发一种基于反应的新型光探针,用于监测不稳定的Fe2+) 池.
- 研究生物铁调节的生理和病理后果.
- 为可视化活细胞中铁的动态提供一种工具.
主要方法:
- 开发了铁探测器1 (IP1) 使用生物灵感,铁介导的氧化C-O键裂解.
- 采用基于光的启动策略进行选择性Fe2+) 检测.
- 应用IP1监测水溶液和活细胞中的不稳定铁池.
主要成果:
- IP1显示了对Fe2+的选择性开启光反应,而不是其他金属离子.
- 在补充或耗尽铁后,可视化细胞中性铁池的变化.
- 检测到内源性,基底水平的不稳定铁池.
- 鉴定了由维生素C和肝素诱导的不稳定铁池的可逆扩张.
结论:
- IP1是第一代用于光Fe2+) 检测的化学工具.
- IP1 允许可视化生物系统中不稳定铁池的动态变化.
- 这个探测器为研究铁信号,压力和开发新探测器提供了一个起点.
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