的小RNA通过劫持宿主RNA干扰通路来抑制植物免疫力
Arne Weiberg1, Ming Wang, Feng-Mao Lin
1Department of Plant Pathology and Microbiology, University of California, Riverside, CA 92521, USA.
概括
Botrytis cinerea使用小RNAs (Bc-sRNAs) 来抑制植物免疫基因,从而使灰菌疾病成为可能. 这种跨王国RNA干扰突出了先进的真菌毒性策略.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- Botrytis cinerea会在200多种植物中引起灰菌病.
- 病原体经常使用复杂的机制来克服宿主防御.
研究的目的:
- 调查Botrytis cinerea小RNAs (Bc-sRNAs) 在植物免疫中的作用.
- 阐明Bc-sRNA与宿主RNA干扰 (RNAi) 途径相互作用的机制.
主要方法:
- 对针对植物免疫基因的Bc-sRNAs的分析.
- 使用Arabidopsis ago1突变物来评估易感性.
- 使用 Botrytis cinerea dcl1 dcl2 双重突变来评估病原性.
主要成果:
- 发现特定的Bc-sRNA可以使阿拉比多普西斯和番茄免疫基因沉默.
- Bc-sRNAs通过与Argonaute 1 (AGO1) 相互作用来劫持宿主RNA干扰机制.
- 阿拉比多普西斯ago1突变体对B. cinerea的敏感性降低,而dcl1 dcl2突变体对病原性降低.
结论:
- 黄菌使用毒性小RNA效应剂来抑制宿主免疫力.
- 自然发生的跨王国RNA干扰是B. cinerea的关键毒性机制.
- 这项研究揭示了真菌病原和宿主免疫抑制的先进策略.
相关概念视频
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...


