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Environmental Sampling of Photosynthetic Microbes and Their Viruses: From Field to Lab

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视觉化病毒组装中间体在海洋蓝藻细菌内部

Wei Dai¹, Caroline Fu, Desislava Raytcheva

¹National Center for Macromolecular Imaging, Verna and Marrs Mclean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.

|October 11, 2013

概括

研究人员使用冷电子断层扫描对其宿主Synechococcus内部的蓝 Syn5组件进行了可视化. 这揭示了这些必不可少的海洋病毒的独特的病毒组装中间体和保存的形态遗传途径.

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科学领域:

微生物学微生物学
病毒学病毒学
海洋生物学海洋生物学

背景情况:

蓝藻是关键的光合作用生物,固定了地球碳的很大一部分.
蓝菌是感染蓝菌的病毒,在海洋生态系统和基因转移中发挥关键作用.

研究的目的:

想象和理解蓝 Syn5 在宿主细胞内的复杂成熟过程.
确定不同的组装中间体和Syn5.5的形态遗传途径.

主要方法:

利用Zernike相位对比电子冷图谱 (cryoET) 进行细胞结构的高对比成像.
在各种感染阶段分析受感染的Synechococcus细胞,观察菌体后代的发育.

主要成果:

在受感染的细胞中识别了细胞下部件,如甲状腺膜,碳素体和多核体.
观察到Syn5转体释放脚手架蛋白质,并在基因组包装过程中扩张.
检测到具有尾巴的成熟Syn5粒子,其中一些具有额外的角结构.

结论:

描述了蓝 Syn5 组装的详细形态遗传途径.
突出了这种途径的保存性质,表明复杂生物体的病毒之前的古代起源.