相关实验视频

Updated: May 7, 2026

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

在flg22诱导的Arabidopsis FLS2-BAK1免疫复合体激活的结构基础

Yadong Sun¹, Lei Li, Alberto P Macho

¹School of Life Sciences, Tsinghua University, Beijing 100084, China, and Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.

Science (New York, N.Y.)

|October 12, 2013

概括

植物免疫受体FLS2及其共同受体BAK1结合于细菌的鞭毛蛋白表位体flg22. 结构分析揭示了这些蛋白质如何识别flg22,激活植物免疫力.

科学领域:

植物免疫学植物免疫学
结构生物学是结构生物学.
植物防御的分子机制

背景情况:

植物免疫反应是由检测保存微生物分子的模式识别受体 (PRR) 启动的.
在Arabidopsis中,旗蛋白感知涉及旗蛋白敏感2 (FLS2) 识别旗蛋白表位素flg22.
对于下游的信号传输,FLS2的激活需要共受体BRASSINOSTEROID INSENSITIVE 1-关联激酶1 (BAK1).

研究的目的:

确定FLS2-BAK1复合体结合到flg22.22的晶体结构.
阐明FLS2-BAK1免疫受体复合体对flg22识别的分子基础.
了解植物免疫受体激活的最初步骤.

主要方法:

在FLS2和BAK1的X射线晶体学FLS2和BAK1的ectodomains复杂的flg22.
在3.06安格斯特罗姆的高分辨率结构分析.
蛋白质 - 配体相互作用的生物化学分析.

主要成果:

晶体结构显示了flg22的C端段和N端段在FLS2上分别有保存和非保存的结合点.
在flg22结合时,FLS2ectodomain没有经历显著的寡合化或形状变化.
BAK1充当共同受体,直接与FLS2相互作用,并识别FLS2结合flg22的C端.

结论:

这项研究提供了关于FLS2-BAK1复合体如何识别flg22.
这种结构性理解澄清了初始植物免疫受体复合体激活的分子机制.
这些发现为了解植物免疫信号通路提供了基础.

更多相关视频

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

相关概念视频

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...