人体细胞中三维染色体相互作用体的高分辨率地图
Fulai Jin1, Yan Li, Jesse R Dixon
11] Ludwig Institute for Cancer Research, 9500 Gilman Drive, La Jolla, California 92093, USA [2].
Nature
|October 22, 2013
概括
定义人类cis-regulatory序列的目标基因是一项挑战. 使用Hi-C,研究人员绘制了超过一百万个染色体相互作用的地图,揭示了预先存在的循环预测了基因激活,这表明稳定的3D基因组会影响细胞特异性基因调节.
科学领域:
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 识别人类cis-regulatory序列的目标基因是基因组学中的一个重大挑战.
- 基于染色体构造捕获 (3C) 的技术提供了绘制长距离染色体相互作用的策略,但往往缺乏全基因组分辨率和覆盖.
研究的目的:
- 为了生成一个全面的,高分辨率的染色体相互作用地图的人类基因组.
- 揭示染色体组织和动态的一般原则.
- 调查促进剂-增强剂接触在基因调节中的作用.
主要方法:
- 在人类纤维细胞中利用全基因组染色体构造捕获分析方法 (Hi-C).
- 实现了5-10kB的分辨率,绘制了超过100万个长距离染色体相互作用的地图.
- 在响应TNF-α信号传递时,描述了促进剂-增强剂接触的动态.
主要成果:
- 创建了一个全面的色素相互作用地图,具有前所未有的分辨率和覆盖范围.
- 在各种基因组特征中发现了染色质组织的基本原理.
- 观察到TNF-α响应增强剂在信号发送之前与它们的标促进剂预相关.
- 证明了先前存在的染色体循环是基因诱导的强有力的预测因素.
结论:
- 三维色氨酸格局在细胞类型中相对稳定.
- 已建立的染色质循环会通过无处不在的转录因子影响细胞特异性选择和目标基因的激活.
- 预先存在的促进剂-增强剂接触在预测细胞外信号传递时的基因诱导方面发挥着至关重要的作用.
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