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从大肠杆菌的lac UV5促进体开始转录的中间体

D C Straney, D M Crothers

|December 1, 1985

概括

研究人员使用大肠杆菌RNA聚合酶在lac UV5促进器上研究了细菌转录启动. 他们确定了不同的中间复合体,揭示了早期转录步骤的热调节.

科学领域:

分子生物学分子生物学
生物化学生物化学
遗传学是一个遗传学.

背景情况:

细菌转录启动是一个复杂的过程,涉及RNA聚合酶与促进DNA结合.
了解中间步骤对于阐明基因调节机制至关重要.

研究的目的:

描述由大肠杆菌RNA聚合酶在lac UV5促进体的转录启动过程中形成的中间复合体.
研究温度在调节这些早期转录事件中的作用.

主要方法:

使用非自然化的聚烯胺凝电泳来分离转录启动中间体.
用DNAase I足迹,蛋白质子单元分析,RNA含量确定和转录试验进行了表征.

主要成果:

确定了两个不同的"开放"复合体,它们在失败的循环能力上有所不同,但不是DNAase I足迹,它们的比例取决于温度.
发现了三个"启动"的复合体,含有RNA链≥11个核酸,缺少西格玛子单元.

结论:

这项研究提供了详细的分子视图早期转录启动步骤在大肠杆菌的乳酸促进器.
热调节显著影响这些中间复合物的平衡和性质.

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