线粒染色体组织的组织
Natalia Naumova1, Maxim Imakaev, Geoffrey Fudenberg
1Program in Systems Biology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School (UMMS), 368 Plantation Street, Worcester, MA 01605-0103, USA.
概括
研究人员发现了两个不同的3D基因组折叠状态. 甲相染色体表现出一个统一的,位置独立的组织,不像在间相中看到的细胞类型特定模式.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 细胞生物学 细胞生物学
背景情况:
- 一个多世纪以来,线粒染色体的内部组织在很大程度上是未知的.
- 之前对非同步细胞的研究描述了一个分隔式和细胞类型特定的基因组组织.
研究的目的:
- 为了研究整个细胞周期的三维 (3D) 基因组组织.
- 阐明调节元相染色体结构的原则.
主要方法:
- 在整个细胞周期中应用染色体构造捕获技术,特别是5C和Hi-C.
- 使用聚合物模拟来建模和测试不同的组织假设.
主要成果:
- 识别两个不同的3D基因组折叠状态:一个在介面阶段,另一个在元阶段.
- 证明之前描述的分隔式组织是特定于相间的.
- 在基因相中发现一种同质的,位置独立的折叠状态,在所有染色体和细胞类型中一致.
结论:
- 基因相染色体组织遵循一个与基因相之间不同的一般原则.
- 这些数据支持纵向压缩的染色质环线性模型,反驳了元相染色体的经典等级模型.
相关概念视频
The Mitotic Spindle
7.1K
The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
7.1K
The Mitotic Spindle
4.9K
4.9K
Chromosome Structure
22.1K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
22.1K
Chromosome Structure
5.5K
5.5K
Forces Acting on Chromosomes
3.1K
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...
Microtubules and motor proteins exert two types of forces on...
3.1K
Mitosis and Cytokinesis
9.9K
In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
9.9K


