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Updated: May 5, 2026

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
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在脂质双层中对膜蛋白进行纳米化学红外成像

Samuel Berweger1, Duc M Nguyen, Eric A Muller

  • 1Department of Physics and Department of Chemistry, ‡Department of Chemical and Biological Engineering, and §Department of Molecular, Cellular, and Developmental Biology, University of Colorado , Boulder, Colorado, 80309, United States.

Journal of the American Chemical Society
|November 21, 2013
PubMed
概括

红外散射类型扫描近场光学显微镜 (s-SNOM) 实现了20nm分辨率的生物分子成像. 这种振动纳米谱技术为蛋白质分布分析提供了高化学特异性.

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相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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