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相关概念视频

DNA Helicases00:55

DNA Helicases

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DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
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The DNA Replication Fork01:02

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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DNA Replication02:40

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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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在没有键的情况下对DNA复制的结构洞察力.

Karin Betz1, Denis A Malyshev, Thomas Lavergne

  • 1Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, Universität Konstanz , Universitätsstrasse 10, D-78464 Konstanz, Germany.

Journal of the American Chemical Society
|November 29, 2013
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概括
此摘要是机器生成的。

将基因字母扩展到像d5SICS-dNaM这样的不自然基对,可以增强DNA的潜力. 然而,由于聚合酶相互作用和基对互插,它们的复制面临着挑战.

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科学领域:

  • 分子生物学分子生物学
  • 合成生物学 合成生物学
  • 生物化学 生物化学

背景情况:

  • 遗传字母以非自然基对 (UBP) 的扩展有望增加遗传和化学潜力.
  • d5SICS-dNaM对是一种高效复制的UBP,但由于其在自由DNA中的间隔结构,其复制机制仍然不清楚.

研究的目的:

  • 在d5SICS.对面插入dNaMTP时,研究化学前后复合体的形成.
  • 阐明结构动力学和聚合酶相互作用,控制d5SICS-dNaM非自然基对的复制.

主要方法:

  • 在d5SICS.对面插入dNaMTP的前化学复合物的表征.
  • 分析后化学复合体,详细说明非自然基对在插入后的位置和构造.
  • 研究聚合酶活性部位相互作用和核酸结合亲缘关系.

主要成果:

  • 与d5SICSTP插入不同,dNaMTP添加并不能完全诱导一个封闭的聚合酶状态.
  • 插入后,d5SICS-dNaM对通过两种不同的模式在聚合酶活性部位内相互插入,受侧面核酸的影响.
  • 间隔降低了随后正确三酸盐结合的亲和力,阻碍了进一步的原料扩展.

结论:

  • 不自然的d5SICS-dNaM基对的复制受到插入后的间隙和随后需要去间隙和活性位点重新排列的限制.
  • 了解这些结构动态对于优化UBP的复制和推进合成生物学至关重要.