对T细胞抗原受体β链家族的抗体检测单克隆T细胞增殖
Lancet (London, England)
|October 11, 1986
概括
针对T细胞受体Vβ基因段的单克隆抗体可以识别恶性T细胞. 这种方法有助于从组织样本直接诊断T细胞淋巴瘤和白血病.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
背景情况:
- T细胞受体 (TCR) 组件使用类似于免疫球蛋白基因的基因段.
- TCRβ链使用有限的可变 (V) 基因段,可通过单克隆抗体识别.
- 这些抗体定义T细胞种群,并评估T细胞疾病中的单克隆性.
研究的目的:
- 评估抗Vβ单克隆抗体在诊断T细胞恶性瘤中的有用性.
- 为了确定Vβ基因段的使用是否可以在淋巴瘤和白血病中识别恶性T细胞群.
主要方法:
- 测试两种抗Vβ单克隆抗体 (抗HPB-A11和抗Jurkat).
- 对24例病例的分析:20例固体T细胞淋巴瘤和4例T细胞白血病.
- 在组织切片和完整的细胞悬浮液中进行直接诊断.
主要成果:
- 三个T细胞淋巴瘤病例对Vβ抗体呈阳性.
- 两个淋巴瘤与抗Jurkat反应,一个与抗HPB反应.
- 该研究证明了使用Vβ抗体染色来诊断T细胞恶性瘤的可行性.
结论:
- 对Vβ基因段的单克隆抗体是T细胞淋巴瘤和白血病的有效诊断工具.
- 这种方法允许从组织样本直接诊断.
- 该方法有可能监测恶性T细胞群体的治疗反应.
相关概念视频
Hybridoma Technology
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Diversity of Antigen Receptors
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...


