通过合成酵母平台从棕色藻糖中有效地生产乙醇
Maria Enquist-Newman1, Ann Marie E Faust1, Daniel D Bravo1
11] Bio Architecture Lab Inc., 604 Bancroft Way, Suite A, Berkeley, California 94710, USA [2].
Nature
|December 3, 2013
概括
棕色大藻可以用于可持续的生物燃料. 研究人员设计了酵母来有效地从藻类中代谢酸盐和曼尼托尔,从而实现了与传统原料相比的大量乙醇生产.
科学领域:
- 生物技术是生物技术.
- 可再生能源可再生能源是可再生能源.
- 海洋资源 海洋资源
背景情况:
- 越来越多的食品和燃料需求需要可持续的原料.
- 棕色大藻提供了一个有希望的,未充分利用的资源.
- 目前的工业微生物如Saccharomyces cerevisiae缺乏有效的酸盐和曼尼托尔利用途径.
研究的目的:
- 为了设计Saccharomyces cerevisiae,以便有效地利用棕色藻原料.
- 从酸盐和曼尼托尔实现商业规模的生物燃料生产.
- 发现和实施关键藻类糖的新型载体.
主要方法:
- 发现并描述了一种来自Asteromyces cruciatus的新型酸盐单体 (DEHU) 载体.
- 在基因组上将DEHU转运基因和细菌酸盐代谢基因集成到S. cerevisiae中.
- 在S. cerevisiae中放松了本地曼尼托尔代谢途径.
- 调整了工程菌株以适应无氧生长和发酵.
主要成果:
- 设计的S. cerevisiae有效地代谢了DEHU和曼尼托尔.
- 在无氧条件下从这些糖中实现显著的乙醇发酵.
- 获得的乙醇位为4.6% (v/v),产量高达理论最大值的83%.
结论:
- 证明了使用棕色藻作为生物燃料的综合原料的可行性.
- 建立了一个从藻类糖中生产生物燃料和可再生化学品的平台.
- 基于藻类的原料可以与传统的基于耕地资源竞争.
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