相关实验视频

Updated: May 1, 2026

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
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Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

Published on: August 22, 2017

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转移RNA保护16S核糖体RNA中的特定核酸免受化学探针的攻击

D Moazed, H F Noller

    Cell
    |December 26, 1986
    PubMed
    概括

    转移RNA与核糖体结合保护特定的核糖体RNA核酸. 这种保护揭示了对核糖体结构和翻译校对机制的洞察力.

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    Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution10:27

    Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

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    Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells10:34

    Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

    Published on: December 9, 2022

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    相关概念视频

    Types of RNA01:23

    Types of RNA

    Overview
    Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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    RNA Interference01:23

    RNA Interference

    RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
    This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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    Transfer RNA Synthesis02:36

    Transfer RNA Synthesis

    One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
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    Ribosome Profiling02:24

    Ribosome Profiling

    Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
    Applications of ribosome profiling
    Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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    Types of RNA01:20

    Types of RNA

    Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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    Translational Regulation01:29

    Translational Regulation

    Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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