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Updated: Aug 6, 2026

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一种基于 cis-membrane FRET 的方法,用于对细胞表面甘氨酸的蛋白质特异成像

Wei Lin1, Yifei Du, Yuntao Zhu

  • 1Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, and Peking-Tsinghua Center for Life Sciences, Peking University , Beijing 100871, P.R. China.

Journal of the American Chemical Society
|December 7, 2013
PubMed
概括

研究人员开发了一种新方法,通过使用 cis-membrane FRET 成像来可视化活细胞上的特定蛋白质糖化. 这种技术允许详细研究糖对蛋白质的修改如何影响细胞功能.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...