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微RNA-128控制了小鼠的神经元刺激性和运动行为

Chan Lek Tan¹, Joshua L Plotkin, Morten T Venø

¹Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10065, USA.

Science (New York, N.Y.)

|December 7, 2013

概括

微RNA-128 (miR-128) 通过控制神经元刺激性来调节运动行为. 降低的miR-128会导致,而过度表达会减轻小鼠的运动和障碍.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

运动行为控制依赖于可适应的神经网络.
神经元刺激性和信号传递对于运动功能至关重要.
微RNA在神经系统中调节基因表达起着重要的作用.

研究的目的:

研究微RNA-128 (miR-128) 在调节运动行为的作用.
为了确定miR-128如何调节神经元信号和兴奋能力.
探索miR-128在神经疾病中的治疗潜力.

主要方法:

研究了成人神经元中的miR-128表达.
分析了miR-128对离子通道和ERK2信号通路的影响.
使用了具有改变miR-128水平 (减少和过度表达) 的小鼠模型.
评估运动活动,神经元刺激性和疾病表型 (,帕金森氏症).

主要成果:

miR-128抑制离子通道和ERK2信号元件的表达,调节神经元刺激性.
在小鼠中减少的miR-128导致运动活动增加和致命的.
过度表达miR-128减弱了神经元反应和运动活动.
miR-128过度表达减轻了小鼠帕金森氏症样疾病和发作模型中的运动异常.

结论:

miR-128是运动行为和神经元刺激性的关键调节剂.
miR-128的失调与和运动障碍有关.
miR-128显示了治疗和运动障碍的治疗潜力.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...