生物组件的构建块的原子结构和手性
Antoine Loquet1, Birgit Habenstein, Veniamin Chevelkov
1Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chemistry , Am Fassberg 11, 37077 Göttingen, Germany.
Journal of the American Chemical Society
|December 17, 2013
概括
研究人员使用固态NMR确定了III型分泌系统针的构建块的原子结构. 这显示了组件的右侧螺旋结构和高分辨率的子单元接口.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 微生物学 微生物学
背景情况:
- 非共价超分子组件在子单元之间具有独特的接口.
- 在组装状态下的完整构建块的结构通常是未知的.
- 目前的方法,如晶体学和NMR提供单体结构,而不是组装的块结构.
研究的目的:
- 为了确定III型分泌系统 (T3SS) 针的组装状态中的原子分辨率结构.
- 为了阐明T3SS针的超分子接口和螺旋手性.
主要方法:
- 固态核磁共振 (ssNMR) 光谱法用于确定原子结构.
- 原子分辨率结构被安装在现有的电子显微镜密度图中.
- 质量/长度测量用于独立验证.
主要成果:
- 解决了T3SS针的构建块的原子结构,揭示了一个同位四元体子单元复合体.
- 建筑块内的三个独特的超分子接口以原子细节为特征.
- 组件被证实具有右侧螺旋结构,每个子单位的轴向上升被确定.
结论:
- 固态NMR为完整的超分子构建块提供高分辨率的结构信息.
- 确定的结构澄清了T3SS针的组装机制和螺旋手.
- 这种结构的洞察力对于理解T3SS在细菌病变发生过程中的作用至关重要.
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