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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: May 4, 2026

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Published on: March 6, 2017

从单分子力光谱学研究研究的酸脱吸动力学.

Stefanie Krysiak¹, Susanne Liese, Roland R Netz

¹Physik Department and IMETUM, Technische Universität München , 85748 Garching, Germany.

Journal of the American Chemical Society

|December 17, 2013

概括

我们确定了聚氨酸和聚氨酸同类的内在单体脱吸率 (k0). 这项研究揭示了令人惊的低k0值,表明单聚合物从表面脱落的合作性显著.

科学领域:

聚合物物理聚合物物理
表面科学是一门学科.
生物物理学的生物物理.

背景情况:

了解聚合物溶解动力学对于表面相互作用至关重要.
之前的研究缺乏精确的固有单体脱吸速率的确定.

研究的目的:

为了确定聚氨酸和聚氨酸同类的内在单体脱吸率 (k0).
为了研究溶剂质量对聚合物脱动态的影响.

主要方法:

结合实验 (AFM) 和理论 (双态运动聚合物理论) 的方法.
使用恒定拉动速度和等待时间的协议来进行单分子溶解.
系统地改变溶剂质量 (乙醇,盐),以改变脱吸力 (10-90 pN).

主要成果:

毫不含糊地确定了聚合物轮长度 (L),库恩长度 (a),吸附自由能量 (λ) 和内在单体脱吸速率 (k0).
测量出了一个惊人的低内在单体脱吸率 (k0 ≈ 10^5 Hz).
在单个聚合物的脱落过程中表现出显著的合作性.

结论:

低k0值表明强大的合作效应控制单聚合物脱吸.

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Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

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Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

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Last Updated: May 4, 2026

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Published on: March 6, 2017

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

这项研究提供了一种可靠的方法来量化基本的脱吸参数.

这些发现对了解聚合物在接口上的行为有意义.