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在感染期间,Icosahedral细菌 ΦX174形成DNA运输的尾巴
Lei Sun1, Lindsey N Young2, Xinzheng Zhang1
11] Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA [2].
Nature
|December 17, 2013
概括
ΦX174试点蛋白H形成了一个管子,对于传递病毒DNA穿过细菌细胞膜至关重要. 这种结构跨越周等离子体空间,促进基因组转移到细胞质中感染.
科学领域:
- 微生物学 微生物学
- 结构生物学 结构生物学
- 病毒学 病毒学
背景情况:
- 细菌病毒 (细菌体) 采用多种不同的基因组传递策略.
- 没有尾巴的菌体,包括类似ΦX174的菌体,利用独特的机制,涉及DNA运输的试点蛋白质.
- ΦX174试点蛋白H在感染中的确切作用尚不清楚.
研究的目的:
- 为了阐明 ΦX174 试点蛋白 H 在细菌感染中的结构和功能.
- 通过类似于 ΦX174 的大胞体,研究细菌细胞外中DNA传递的机制.
主要方法:
- 确定 ΦX174 H 蛋白的中心域的 2.4 Å 分辨率晶体结构.
- 在试验室内将H蛋白组装成管状结构.
- 基因和生化分析以评估感染性和组装作用.
- 低温电子断层扫描可视化管的形成和功能 in vivo.
主要成果:
- 的 ΦX174 H 蛋白质寡合成一个 170 Å 长的 α-螺旋管.
- 这种H蛋白管跨越了周等离子体空间,并促进了DNA基因组传递到宿主细胞质中.
- 两端的跨膜域将管子固定在细菌细胞膜内.
- 管的中央通道,着特定的氨基酸残留物,对于高效的DNA转移至关重要.
结论:
- 这种 ΦX174 H 蛋白质形成了细菌感染所必需的 DNA 导体.
- 这种试点蛋白质介导管代表了病毒基因组在细菌包裹中传递的新机制.
- 这些发现提供了关于病毒DNA在细胞膜中传输的一般原理的见解.
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