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相关概念视频

DNA Helicases00:55

DNA Helicases

24.9K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
24.9K
Homologous Recombination02:31

Homologous Recombination

65.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
65.5K
DNA Topoisomerases02:02

DNA Topoisomerases

37.5K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
37.5K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

17.2K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
17.2K
The DNA Replication Fork01:02

The DNA Replication Fork

43.1K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
43.1K
The DNA Replication Fork01:02

The DNA Replication Fork

20.3K
No description available
20.3K

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相关实验视频

Updated: Mar 30, 2026

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

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一种发育调节的活动,解开RNA复合体.

B L Bass, H Weintraub

    Cell
    |February 27, 1987
    PubMed
    概括

    在Xenopus胚胎中的反意义RNA技术是暂时的,这是由于RNA:RNA杂交解活动. 这种活动从卵细胞到胚胎生成都存在,阻碍了基因调节的稳定混合体形成.

    科学领域:

    • 发展生物学 发展生物学
    • 分子生物学分子生物学
    • 在RNA生物学,RNA生物学.

    背景情况:

    • 反理性RNA技术是基因调节的工具.
    • RNA:RNA混合物是基因调节中的关键中间体.
    • 这些混合物的稳定性对于抗意义RNA的有效性至关重要.

    研究的目的:

    • 在发育中的Xenopus胚胎中研究感觉:反感觉RNA杂交的稳定性.
    • 为了确定可能限制抗意义RNA技术在这个模型生物体中的有效性的因素.

    主要方法:

    • 在Xenopus胚胎中利用反意义RNA技术.
    • 分析了RNA:RNA杂交的形成和稳定性.
    • 在Xenopus卵细胞和胚胎的S100提取物中表现出RNA解开活性.

    主要成果:

    • 感觉:反感觉RNA杂交物形成,但在Xenopus胚胎中是暂时的.
    • 在卵细胞中检测到一种RNA:RNA混合解活动,并在成熟和胚胎发生过程中增加.
    • 这种活性在S100提取物中具有特征,并且被证明是对重复RNA的特异性.

    结论:

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    Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
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    A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases

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    • 一种强大的RNA:RNA杂交解活动限制了Xenopus. antisense RNA杂交的稳定性.
    • 这种活动是发育调节的,存在于 oogenesis 通过胚胎生成.
    • 这些发现表明,在Xenopus早期发育过程中应用直接反意义RNA策略存在挑战.