在支持的脂质双层膜中进行芯片电泳,使用低电位实现
Jasper van Weerd1, Sven O Krabbenborg, Jan Eijkel
1Molecular nanoFabrication group and ‡BIOS lab-on-a-chip group, MESA+ Institute for Nanotechnology, University of Twente , P.O. Box 217, 7500 AE Enschede, The Netherlands.
Journal of the American Chemical Society
|December 19, 2013
概括
一种新的微型支持脂质双层 (SLB) 电泳方法使用铁衍生物来实现低压操作. 这种技术避免了水电解,保持了SLB完整性和生物分子功能,用于分析设备的开发.
科学领域:
- 生物物理学的生物物理.
- 分析化学 分析化学
- 电化学 电化学 电化学
背景情况:
- 支持的脂质双层 (SLBs) 对于模仿生物传感中的细胞膜至关重要.
- 传统的电泳法通常需要高潜力,导致水电解和有限的操作时间等问题.
- 开发低电位电泳技术对于保持SLB完整性和生物分子功能至关重要.
研究的目的:
- 开发一种在低电位下运行的微支持脂质双层 (SLB) 电泳法.
- 为了提高SLB电泳的稳定性和效率,用于分析应用.
- 调查电化学介质的使用,以促进低压操作.
主要方法:
- 一个微流体装置被用于支持的脂质双层 (SLB) 电泳.
- (基甲基) - 铁 (FcCH2OH) 被用作电化学介质,以使低应用电位的反应成为可能.
- 在不同的电场下分析了染料修饰脂质和链素的电泳性移动性.
主要成果:
- 开发的方法在低电位 (0.25-1.2V) 上有效运行,运行时间延长.
- 添加FcCH2OH可以防止水电解,泡形成以及pH和温度的变化.
- 实现了均的电场,导致充电的染料修饰脂质的快速,可逆和强大的积累.
- 已经证明了斯特雷普塔维丁流动性的调节,这表明该方法对生物分子分析的实用性.
结论:
- 这种低电位SLB电泳法为生物分子分析提供了稳定高效的平台.
- 使用FcCH2OH作为电化学媒介克服了传统电泳的局限性.
- 这种技术对微流体分析装置的进步具有重大潜力.
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