不同代的多基化阿达曼坦基的树枝表现出高细胞吸收率,而不会引发细胞毒性
Maxime Grillaud1, Julie Russier, Alberto Bianco
1CNRS, Institut de Biologie Moléculaire et Cellulaire, Immunopathologie et Chimie Thérapeutique , Strasbourg, France.
Journal of the American Chemical Society
|December 21, 2013
概括
研究人员为生物医学应用开发了称为HYDRAmers的新型,无毒的多化树枝. 这些基于阿达曼坦的纳米结构高效地进入细胞,显示了基因和药物输送系统的前景.
科学领域:
- 纳米技术 纳米技术
- 材料科学 材料科学 材料科学
- 生物医学工程 生物医学工程
背景情况:
- 像聚乙烯胺 (PEI) 这样的多化树突体显示出细胞传递的潜力,但往往表现出毒性.
- 这种毒性限制了它们作为基因或药物输送载体的临床应用.
- 需要新的树突纳米结构来克服这些局限性.
研究的目的:
- 设计和合成具有降低细胞毒性的新型多化树 (HYDRAmers).
- 为了评估这些新型树枝的细胞吸收效率.
- 探索它们作为生物医学应用的安全有效纳米载体的潜力.
主要方法:
- 合成第一和第二代基于阿达曼坦的树枝与乙烯糖醇链接剂和氨/瓜尼尼外围群体.
- 对RAW 264.7和HeLa细胞系的细胞毒性评估.
- 细胞内化研究使用5光标签和点击化学.
主要成果:
- 合成的HYDRAmers在测试的细胞系中没有显著的细胞毒性.
- 所有的树枝都表现出高效的细胞吸收,根据世代和外围群体而有所不同.
- 点击化学启用了有效的光标签和跟踪树突内化.
结论:
- 聚化HYDRAmers代表了一个有前途的类别的无毒纳米结构.
- 它们高效的细胞透表明,它们有可能成为基因和药物输送的先进载体.
- 对HYDRAmers的进一步研究可以促进纳米医学的发展.
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