miR-9a通过缓冲转录因子来最大限度地减少基因组多样性的表型影响
Justin J Cassidy1, Aashish R Jha2, Diana M Posadas1
1Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA.
Cell
|December 24, 2013
概括
基因组多样性影响细胞行为,但microRNA-9a (miR-9a) 通过调节无意识表达来抑制这些影响. 减少miR-9a允许基因组变异更显著地影响细胞表型.
科学领域:
- 遗传学 是一个遗传学.
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 基因表达必须对遗传和环境变化有强大的抵抗力.
- 基因组多样性,即人类DNA的0.5%-1%变异,可以改变基因调节网络和细胞行为.
- 了解缓冲基因组变异的机制对于细胞功能至关重要.
研究的目的:
- 为了研究基因组多样性如何影响Drosophila proneural网络.
- 确定微RNA-9a (miR-9a) 在调节基因组多样性的影响中的作用.
- 探索miR-9a,无意义表达和表型变异之间的关系.
主要方法:
- 在Drosophila.多代选择实验.
- 对miR-9a介导的Senseless转录因子调节的分析.
- 全基因组测序以确定影响表型的遗传基因位置.
主要成果:
- miR-9a在Drosophila前神经网络中抑制了基因组多样性的表型效应.
- 降低miR-9a水平增加了基因组变异对细胞行为的影响.
- 基因组位点和proneural基因内的序列变异被确定为这种变异的贡献者.
结论:
- 微RNA-目标相互作用,特别是无意识的miR-9a调节,是减轻基因组多样性对细胞行为影响的关键.
- 这种调节机制为基因调节网络提供了强度.
- 这些发现强调了微RNA在保持细胞一致性方面的重要性,尽管存在遗传变异.
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