二维总频率生成揭示了与表面结合的的结构和动态
Jennifer E Laaser1, David R Skoff, Jia-Jung Ho
1Department of Chemistry and ‡Department of Materials Science and Engineering, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
Journal of the American Chemical Society
|December 31, 2013
概括
二维总频生成 (2D SFG) 光谱学成功地描述了表面上的结构. 这种技术揭示了详细的体构造,克服了表面结合生物分子的传统方法的局限性.
科学领域:
- 表面科学是一门学科.
- 频谱学是一种光谱学.
- 生物物理学的生物物理.
背景情况:
- 通过蛋白质和多来功能化无机表面对于生物传感器和先进材料等应用至关重要.
- 由于接口复杂性和溶解效应,对这些表面结合的生物分子的结构进行表征是传统光谱技术的挑战.
研究的目的:
- 开发和应用一种新的光谱方法,用于在无机表面上对单层的详细结构分析.
- 证明二维总频生成 (2D SFG) 光谱在阐明表面固定的构造方面的能力.
主要方法:
- 实现一个中红外脉冲塑造器与一个五秒钟SFG光谱仪,以获取二维SFG光谱.
- 分析2D SFG光谱线形状,无和变化和振动寿命,以推断分子结构.
- 将2D SFG光谱与传统的里埃变换红外光谱 (FTIR) 进行比较,以突出优势.
主要成果:
- 2D SFG光谱显示,黄金表面上的单层采用了主要的α螺旋和直立形状.
- 该技术成功地通过2D SFG光谱的交叉峰检测到随机卷轴残留物,尽管它们的同位分布.
- 在FTIR中掩盖结构信息的溶解诱导的频率转移被2D SFG方法所克服.
结论:
- 2D SFG光谱是一种强大的工具,用于描述表面结合和蛋白质的结构.
- 已建立的可溶性蛋白质的结构-2D红外光谱关系可以通过2D SFG扩展到表面绑定系统.
- 这一进步促进了用于功能化接口和生物分子应用的合理体设计.
更多相关视频
11:44Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities
Published on: October 2, 2018
10.0K
05:44Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy
Published on: March 6, 2017
7.6K
相关概念视频
01:33Peptide Identification Using Tandem Mass Spectrometry
6.2K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.2K
01:12Two-Dimensional (2D) NMR: Overview
1.6K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
1.6K