相关实验视频

Updated: May 4, 2026

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Published on: August 9, 2022

插电式光体扩展了菜的光谱特性

Wenjiao Song¹, Rita L Strack, Nina Svensen

¹Department of Pharmacology, Weill Medical College, Cornell University , New York, New York 10065, United States.

Journal of the American Chemical Society

|January 8, 2014

概括

现在可以使用新的"插即用"光体来成像西班牙菜2RNA光体. 这些新的菜2-光体复合体为使用标准显微镜过器对活细胞RNA成像提供了改进的光谱特性.

科学领域:

分子生物学分子生物学
生物化学生物化学
生物物理学的生物物理.

背景情况:

菜和菜2是RNA的阿普坦,使得基因编码的RNA光.
菜2-DFHBI复合体允许活细胞对RNA定位的成像,但具有光谱限制.
现有的Spinach2光是蓝绿色的,对于常见的显微镜过器来说是不理想的.

研究的目的:

为了描述菜2光结合的结构要求.
开发与菜相容的新型光体2.
为了增强基于菜2的RNA成像的光谱特性.

主要方法:

菜2-化物相互作用的结构分析.
新型光体的合成和表征.
活细胞成像实验使用菜2标记的RNA和新的光体.

主要成果:

确定了菜2光结合的关键结构特征.
开发了新的光体,可以结合并激活菜2的光.
与GFP和YFP过器集实现了更好的光兼容性.
在活细胞中成功成像了带有Spinach2标记的RNA,其光谱特性得到了增强.

结论:

新型光体扩大了菜2 RNA 吸收体对活细胞成像的效用.
这种"插即用"的光体系统可以为各种实验需求提供可调节的光谱特性.
增强的菜2-光体复合物促进了光显微镜的更广泛采用.

更多相关视频

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Published on: January 2, 2020

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

相关概念视频

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.