使用抗体催化水氧化途径检测抗体的通用平台
M Elizabeth Welch1, Nicole L Ritzert, Hongjun Chen
1Department of Chemistry and Chemical Biology, Cornell University , 122 Baker Laboratory, Ithaca, New York 14853, United States.
Journal of the American Chemical Society
|January 14, 2014
概括
一个新的生物传感器使用抗体催化水氧化途径 (ACWOP) 检测传染病抗体. 这种多功能,便携式设备为全球卫生安全提供了临床相关水平内的快速,可靠的检测.
科学领域:
- 生物医学工程 生物医学工程
- 传染病诊断 传染病诊断 传染病诊断
- 生物传感器技术技术
背景情况:
- 传染病和流行病对全球健康构成重大威胁.
- 准确和快速的诊断对于疾病监测和控制至关重要.
- 现有的诊断方法可能缺乏广泛使用所需的速度,可移植性或特异性.
研究的目的:
- 开发一种新的生物传感器,用于检测与传染病相关的抗体.
- 创建一个多功能平台,能够检测跨多个物种的抗体.
- 建立一个快速,便宜,便携,可靠的诊断工具.
主要方法:
- 利用基于抗体催化水氧化途径 (ACWOP) 的电化学生物传感器.
- 采用聚合物刷修改表面进行高亲和抗体-哈普结合.
- 检测到由ACWOP产生的过氧化,通过Resorufin/Amplex Red进行信号调解.
主要成果:
- 通过ACWOP生物传感器成功证明了抗体的检测.
- 在临床相关水平内的度中检测到抗体.
- 使用模型抗DNP抗体对生物传感器平台进行了表征.
结论:
- 开发的生物传感器平台有效检测传染病相关抗体.
- 基于ACWOP的生物传感器显示出作为通用,灵敏和快速诊断工具的希望.
- 这项技术有可能提高全球传染病监测和流行病准备能力.
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