概括
血小板衍生生长因子 (PDGF) 和多瘤中T抗原 (MTAg) 触发了85kd蛋白的氨酸酸化,增加了酸丁醇 (PI) 激酶活性. 这种脂蛋白是氨酸激酶的基质.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 氨酸酸化在细胞信号通路中起着至关重要的作用.
- 血小板衍生生长因子 (PDGF) 是一种强大的线粒原体,参与细胞生长和分化.
- 多瘤中T抗原 (MTAg) 是一种病毒性瘤蛋白,可以诱导细胞转化.
研究的目的:
- 研究氨酸酸化在细胞对PDGF刺激和MTAg转化反应中的作用.
- 识别与这些细胞事件相关的蛋白质和酶活性.
主要方法:
- 用一种抗氨酸 (P-tyr) 的抗体进行免疫沉.
- 检查了3T3细胞中的蛋白质酸化和酸丁醇 (PI) 激酶活性.
- 分析的细胞被PDGF刺激并被MTAg,v-fms,v-sis和SV40 T抗原转化.
主要成果:
- 在PDGF刺激或MTAg转化时观察到85kd蛋白的快速氨酸酸化和PI激酶活性增加.
- 在由v-fms和v-sis转换的细胞中,85kd的蛋白和PI激酶活性也升高,但不是SV40 T抗原.
- 在净化过程中发现了氨酸化85kd蛋白和PI激酶活性之间的强烈相关性.
结论:
- 85kd的蛋白,可能是酸丁醇 (PI) 激酶,是PDGF受体和MTAg/pp60c-src氨酸激酶活动的关键基质.
- 这些发现阐明了一种信号通路,涉及氨酸酸化和PI激酶激活,以应对生长因子和病毒coproteins.
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