对细胞外ATP的植物受体的鉴定
Jeongmin Choi1, Kiwamu Tanaka, Yangrong Cao
1Divisions of Biochemistry and Plant Sciences, Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.
Science (New York, N.Y.)
|January 18, 2014
概括
植物利用细胞外腺5'-三酸盐 (ATP) 进行生长和应激反应. 研究人员确定了DORN1蛋白质是Arabidopsis细胞外ATP的关键受体,对于植物信号和抗压能力至关重要.
科学领域:
- 植物生物学 植物生物学
- 分子信号传输的方法
- 生物化学 生物化学
背景情况:
- 细胞外腺5-三酸盐 (ATP) 是哺乳动物的一个重要信号分子,通过P2型纯感受体起作用.
- 植物缺乏直接的ATP受体,但利用细胞外ATP进行生长,发育和应激反应.
研究的目的:
- 确定植物用来感知细胞外ATP的分子机制.
- 描述鉴定的受体在植物反应中的作用.
主要方法:
- 基因查以识别Arabidopsis中的ATP不敏感突变体.
- 生物化学分析以确定已识别的蛋白质的ATP结合亲和力.
- 生理学测试以评估突变者对ATP和受伤的反应.
主要成果:
- 鉴定了对ATP不敏感的突变Dorn1,该突变在莱克受体激酶I.9 (DORN1) 中有缺陷.
- DORN1表现出高亲和度的ATP结合 (Kd=45.7 ± 3.1 nM). 在这种情况下,DORN1具有高亲和度的ATP结合.
- DORN1对于ATP诱导的信号传递,MAPK激活和基因表达至关重要,其宫外表达增强了伤口反应.
结论:
- 在Arabidopsis中,DORN1作为细胞外ATP的高亲和感受体.
- DORN1对于调解植物对细胞外ATP的反应至关重要,特别是在抗压力方面.
- 这一发现为了解植物细胞外ATP信号提供了分子基础.
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