在与乙型肝炎核心蛋白融合后,增强了类表位的免疫性
B E Clarke1, S E Newton, A R Carroll
1FMD Division, Wellcome Biotechnology Ltd, Beckenham, Kent, UK.
Nature
|November 2, 1987
概括
使用病毒蛋白碎片的合成疫苗显示出有希望. 一个呈现乙型肝炎核心抗原上表位的新系统实现了与整个FMDV颗粒相比的免疫性.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 疫苗开发 疫苗开发
背景情况:
- * 合成疫苗可以利用特定的病毒蛋白区域 (表位) 作为免疫原体.
- * 足口病病毒 (FMDV) VP1蛋白的序列 (141-160) 可以引起中和抗体.
- *以前使用单独的或与β-galactosidase结合的的尝试与整个病毒相比显示出有限的免疫性.
研究的目的:
- * 开发一种表达系统,用于制造强效的合成疫苗.
- *使用类似病毒的复合体,在高密度下呈现免疫原性表位.
- * 增强病毒表位的免疫性.
主要方法:
- *构建一种表达系统,将免疫原性表位物与载体蛋白联系起来.
- *利用乙型肝炎核心抗原作为载体蛋白来形成类似病毒的复合体.
- *评估这些新型结构的免疫性.
主要成果:
- *开发的表达系统成功地在高密度下呈现了表位.
- * 工程病毒样复合体的免疫性接近原生FMDV颗粒的免疫性.
- * 这种方法显著改善了免疫反应,而不是以先前的基方法.
结论:
- * 一种新型的类似病毒的粒子系统有效地呈现病毒表位.
- * 这种系统增强了合成疫苗的免疫性.
- *这种方法显示出开发下一代病毒疫苗的潜力.
相关概念视频
Cell-mediated Immune Responses
Overview
Antigens Involved in Adaptive Immunity
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Cytotoxic T Cells-mediated Immune Response
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Immune Response Against Viral Pathogens
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
Tumor Immunotherapy
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.


