不同的mRNA稳定性控制了多基斯特龙操作子内的相对基因表达
S F Newbury1, N H Smith, C F Higgins
1Department of Biochemistry, University of Dundee, Scotland.
Cell
|December 24, 1987
概括
使者RNA (mRNA) 的稳定性控制了细菌操作子中的基因表达. REP序列稳定了上游mRNA,显著影响了蛋白质合成和基因调节.
科学领域:
- 分子生物学分子生物学
- 细菌遗传学 细菌遗传学
- 基因规则 基因规则
背景情况:
- 多基斯特龙操作子允许在细菌中协调基因表达.
- mRNA稳定性是影响蛋白质水平的关键因素.
- REP 序列是保存在的,在基因间区域中发现的反转重复.
研究的目的:
- 为了研究REP序列在E. colimalEFG操作体内的mRNA稳定性和基因表达中的作用.
- 确定REP序列删除对male mRNA水平和male蛋白质合成的影响.
主要方法:
- 在大肠杆菌染色体malEFG操作子中的REP序列的删除突变发生.
- 对male mRNA稳定性的分析和male蛋白水平的量化.
主要成果:
- 删除REP序列破坏了上游malE mRNA的稳定.
- 丢失REP序列导致MalE蛋白合成显著减少9倍.
- 一个单个REP序列足以稳定上游mRNA.
结论:
- REP序列在稳定多基斯特龙操作子内的上游mRNA中起着至关重要的作用.
- 由REP序列介导的mRNA稳定性是控制相对基因表达的关键机制.
- REP序列的流行表明这种调节机制在细菌中广泛存在.
相关概念视频
01:58Cell Specific Gene Expression
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
01:23Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...