刺激触发的命运转化,体细胞转化为多能细胞
Haruko Obokata1, Teruhiko Wakayama2, Yoshiki Sasai3
11] Laboratory for Tissue Engineering and Regenerative Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA [2] Laboratory for Cellular Reprogramming, RIKEN Center for Developmental biology, Kobe 650-0047, Japan [3] Laboratory for Genomic Reprogramming, RIKEN Center for Developmental biology, Kobe 650-0047, Japan.
Nature
|January 31, 2014
概括
科学家们发现了刺激触发的多能性 (STAP) 获取,这是一种将哺乳动物体细胞重新编程成多能细胞的新方法,仅使用外部刺激,如低pH压力因素. 这种突破性的技术绕过了对核转移或转录因子的需求.
科学领域:
- 细胞生物学 细胞生物学
- 发育生物学是发展生物学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 细胞重编程通常涉及核转移或引入特定的转录因子.
- 哺乳动物体细胞具有稳定的表观遗传状态,使重编程具有挑战性.
研究的目的:
- 报告一个新的细胞重编程现象,刺激触发的多能性 (STAP) 的获取.
- 研究STAP在哺乳动物体细胞中的机制和潜在应用.
主要方法:
- 向哺乳动物体细胞 (淋巴细胞) 施加强烈的外部刺激,特别是短暂的低pH压力因素.
- 利用实时成像和基因重排分析来追踪细胞变化.
- 评估多能性基因调节区域中的DNA甲基化模式.
- 进行胚胎囊注射实验,以评估发育潜力.
主要成果:
- 在没有核转移或转录因子的情况下,STAP成功地将哺乳动物体细胞重新编程成多能细胞.
- 证据表明重新编程,而不是选择,是机制,实时成像和基因分析证实了这一点.
- 在多能性基因区域中,STAP细胞表现出减少的DNA甲基化.
- 通过STAP细胞,可以有效地促进基马体胚胎和后代的生殖系传播.
- 强大的可扩展多能细胞系来自STAP细胞.
结论:
- 环境线索可以显著改变哺乳动物细胞中的表观遗传命运决定.
- STAP是一种独特而高效的方法,可以从体细胞中产生多能细胞.
- 这一发现为再生医学和理解细胞可塑性开辟了新的途径.
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