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在人类转录基因组中RNA二级结构的风景和变化
Yue Wan1, Kun Qu2, Qiangfeng Cliff Zhang3
11] Howard Hughes Medical Institute and Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California 94305, USA [2] Stem Cell and Development, Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672 [3].
Nature
|January 31, 2014
概括
RNA二次结构 (RSSs) 携带重要的基因表达信息,其中大多数被编码在RNA序列中. 遗传变异可以改变这些结构,影响整个基因组的基因调节.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物信息学是一种生物信息学.
背景情况:
- RNA结构是蛋白质编码序列之外的基因信息的关键层.
- 了解RNA二次结构 (RSS) 以及序列变异如何影响它们对于破译基因表达调节至关重要.
研究的目的:
- 在人类编码和非编码RNA中绘制RNA二次结构 (RSS) 的景观和变异.
- 研究遗传变异对RSS的影响及其在基因调节中的作用.
主要方法:
- 在人类家族三组中分析RNA二次结构.
- 原生细胞RNA与重新折叠的净化RNA的比较.
- 影响RSSs的转录单核酸变体 (SNVs) 的识别和表征.
主要成果:
- 人类RSS的综合地图被生成,揭示了功能元素的独特签名,如开放的阅读框架和拼接连接.
- 大多数RSS信息都在RNA序列中内在编码.
- 发现超过1900个转录的SNVs改变了局部RNA结构,具有特定的序列和间距规则来规范它们的影响.
- 有证据表明,在数千个基因组位点,对某些结构变异 ("riboSNitches") 产生选择性压力,这表明对特定RNA形状的选择.
结论:
- RNA二次结构在基因调节中起着重要作用,序列变异有助于这种调节.
- 该研究提供了关于RNA结构变化的功能影响对微RNA结合,RNA结合蛋白相互作用和其他调节过程的见解.
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