2微米圈基因表达的自我调节提供了维持稳定的等离子体拷贝水平的模型
T Som1, K A Armstrong, F C Volkert
1Department of Molecular Biology, Princeton University, New Jersey 08544.
Cell
|January 15, 1988
概括
酵母的2微米等离子体通过基因调节保持稳定的拷贝数. 等离子体蛋白质REP1和REP2抑制FLP复合酶基因和REP1基因转录,确保等离子体的稳定性.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 2微米的等离子体是一个高复制数的酵母病例,缺乏宿主选择性优势.
- 了解其稳定维护机制对于酵母生物技术和基因工程至关重要.
研究的目的:
- 阐明酵母2微米等离子体稳定复制控制的监管机制.
- 为了研究等离子体编码蛋白在调节基因表达和拷贝数量的作用.
主要方法:
- 对关键2微米等离子体基因的基因表达水平的定量分析.
- 检查基因表达在存在或缺少特定的等离子体编码蛋白质.
- 评估REP1和REP2蛋白对FLP和REP1基因转录的影响.
主要成果:
- 由于REP1和REP2蛋白质的联合作用,FLP复合酶基因表达被抑制了100倍以上.
- REP1和REP2蛋白也表现出自我抑制,抑制了REP1基因的转录.
- 这些调节相互作用形成了一个反循环,对于塑体复制控制至关重要.
结论:
- 涉及REP1和REP2蛋白质的调节网络积极控制2微米等离子体基因表达和拷贝数.
- 这种反机制确保了高但稳定的等离子体水平,而不会赋予宿主选择性优势.
- 这些发现为酵母质粒稳定性和基因调节提供了一致的模型.
相关概念视频
Regulation of Expression Occurs at Multiple Steps
20.2K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
20.2K
Prokaryotic Transcriptional Activators and Repressors
20.1K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
20.1K
Maintenance of the ES Cell State
1.9K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
1.9K
Coordination of Gene Expression Processes in Bacteria
1.1K
The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
1.1K
Constitutive and Regulated Gene Expression
1.9K
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
1.9K
Operon Model
2.5K
The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
2.5K


