相关实验视频
Updated: May 3, 2026

09:40
Detection of True IgE-expressing Mouse B Lineage Cells
Published on: December 1, 2014
10.5K
通过IgE的高亲和感受体对细胞反应进行分子编辑
Ryo Suzuki1, Sarah Leach, Wenhua Liu
1Laboratory of Molecular Immunogenetics, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, MD 20892, USA.
概括
巨细胞中的细胞表面受体 (IgE) 信号传递因刺激强度而异. 低亲和度刺激会改变受体行为和信号通路,影响免疫反应和炎症.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 细胞表面受体调解细胞对外部刺激的反应.
- 巨细胞上免疫球蛋白E (IgE) 的高亲和受体对于过敏反应和免疫监测至关重要.
- 众所周知,刺激的强度会影响细胞信号通路,但确切的机制仍然不完全理解.
研究的目的:
- 研究IgE受体如何区分高和低亲和度刺激.
- 为了阐明下游信号事件和受刺激强度调节的效应器功能.
- 了解差异信号对免疫细胞招募和炎症的影响.
主要方法:
- 用对IgE受体具有不同亲和力的配体刺激乳腺细胞.
- 对受体酸化,聚类,移动性和分布的分析.
- 研究涉及Src家族激酶 (Fgr) 和适应蛋白 (LAT1,LAT2) 的信号通路.
- 测量信号传递,巨细胞脱粒化和化学激素的产生.
主要成果:
- 高和低亲和度刺激都诱导了类似的IgE受体酸化.
- 低亲和度刺激导致了不同的受体聚类,移动性和分布模式.
- 观察到与Fgr的受体关联以及从LAT1到LAT2的信号转移与低亲和度刺激.
- 降低了LAT1依赖的脱粒信号,而增加了LAT2介导的化学激素产生.
结论:
- IgE受体表现出不同的下游信号结果,基于刺激亲和力,而不仅仅是酸化水平.
- 信号分子的差异招募 (Fgr,LAT2) 和改变的信号传播决定了效应器的功能.
- 这种IgE受体的刺激歧视微调瘤细胞的反应,影响炎症过程和免疫细胞的招募.
相关概念视频
Antibody Structure
51.8K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
51.8K
Allergic Drug Reactions
1.6K
Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing...
1.6K
Receptor Downregulation in MVBs
2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.0K
Allergic Reactions
26.4K
Overview
26.4K
Diversity of Antigen Receptors
2.1K
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
2.1K
B Cell Activation and Differentiation
14.4K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
14.4K

