在纤维细胞髓分裂过程中改变了pp60c-src的酸化和激活
I Chackalaparampil1, D Shalloway
1Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Cell
|March 25, 1988
概括
在线粒分裂过程中,pp60c-src经历新型酸化,增强其激酶活性4-7倍. 这种独立于Tyr 416/527的修改表明pp60c-src调节了线粒分裂特异性事件.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- pp60c-src是一种非受体氨酸激酶,参与细胞信号传递.
- 分解涉及复杂的调节机制,控制细胞分裂.
研究的目的:
- 为了研究pp60c-src在线粒分裂过程中的修饰和活性.
- 为了确定pp60c-src是否在线粒分裂特异性事件中起作用.
主要方法:
- 使用了NIH 3T3细胞过度表达c-src.
- 在线化过程中分析了pp60c-src酸化部位 (三氨酸,血清氨酸).
- 评估了体外激酶活性和类分析.
- 用于内源性pp60c-src和pp60v-src的电泳运动阻滞剂.
主要成果:
- 超过一半的pp60c-src在线粒分裂期间在其氨基末端区域通过酸化氨酸和氨酸被修饰.
- 线性pp60c-src表现出4至7倍增强的体外激酶活性.
- 修改是独立于Tyr 416/Tyr 527酸化的.
- 内源的pp60c-src和pp60v-src在线粒分裂过程中表现出类似的修饰.
- 增强的活动和修改在细胞分裂附近得到解决.
结论:
- pp60c-src受益于线粒分裂的特定调节.
- 在线分裂期间增强的pp60c-src激酶活性表明它在调节细胞分裂事件中的作用.
- 这些发现突显了pp60c-src信号传递和线粒细胞进展之间的潜在联系.
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