在光系统I中光化学能量转换的长波长极限
Eberhard Schlodder1, Friedhelm Lendzian, Jenny Meyer
1Max-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität Berlin , Strasse des 17. Juni 135, 10623 Berlin, Germany.
Journal of the American Chemical Society
|February 13, 2014
概括
光系统I (PS I) 可以吸收超过800nm的远红光来驱动光化学,即使在长波长叶绿素吸收之外. 这通过直接激发电荷转移状态来发生,从而扩展PS I.
科学领域:
- 光合作用研究研究光合作用.
- 生物物理学的生物物理.
- 植物科学 植物科学
背景情况:
- 光系统I (PS I) 使用长波长的叶绿素 (LWC) 来吸收高达750nm的光,用于光化学.
- 在PS I中光吸收的光谱极限对于理解能量转换效率至关重要.
研究的目的:
- 为了研究PS I中远红光引起的光化学电荷分离的机制,远红光超出800nm.
- 为了确定LWC是否负责在波长>800 nm的电荷分离.
- 为了确定负责远红色光吸收的吸光物种的性质.
主要方法:
- 在295K到5K的温度下对来自Thermosynechococcus elongatus和Arthrospira platensis的PSI核心复合物的光谱分析 (吸收和EPR).
- 使用特定波长 (754,785和808纳米) 选择性激发PSI.
- 对P700氧化和电荷分离中间体的监测.
主要成果:
- 在PS I中,光化学电荷分离是由波长>800nm的光诱导的,在整个研究温度范围内.
- 在754nm,785nm和808nm激发时观察到P700氧化和P700(+•) FA/B(-•) 的形成.
- 观察到的现象与LWC含量无关,排除了LWC作为这些远红波长的主要吸收器.
结论:
- 在波长>800 nm的PS I中,电荷分离不受LWC的介导.
- 这些发现强烈地表明,PS I反应中心内的电荷转移 (CT) 状态的直接激发是负责的.
- 这种机制将PS I的功能光谱范围扩展到LWC吸收之外,对其他光活性系统有潜在的影响.
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