诺斯胺不是一个自我产生的触发生物膜拆卸的触发器
Laura Hobley1, Sok Ho Kim2, Yukari Maezato2
1Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee DD15EH, UK.
Cell
|February 18, 2014
概括
诺斯胺是一种聚胺,不像之前所建议的那样有助于Bacillus subtilis生物膜的形成. 相反,它不在生物膜中,并且可以在更高度下抑制生长.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细菌细菌生物膜的形成需要精子胺.
- 一项先前的研究表明,诺斯胺有助于B. subtilis生物膜的形成.
- 这项研究调查了诺斯胺在B. subtilis生物膜中的作用.
研究的目的:
- 为了调查诺斯米丁在Bacillus subtilis生物膜形成中的实际作用.
- 确定诺斯胺对B. subtilis生长和生物膜的存在和影响.
主要方法:
- 在不同度的外源诺斯珀米丁下培养细菌细菌.
- 在B. subtilis生物膜中量化诺斯胺的含量.
- 评估诺斯胺对浮游生物生长和生物膜形成的影响.
- 在B. subtilis.中分析诺斯胺生物合成途径基因的存在.
主要成果:
- 与之前的发现相反,外源性诺斯胺高达200μM可促进生物膜的形成.
- 在野生型B. subtilis生物膜中,诺斯胺在所有阶段都无法检测到.
- 高度的外源诺斯胺抑制了浮游生物的生长和生物膜的形成.
- 细菌细菌缺乏Vibrio cholerae中发现的诺斯胺生物合成途径.
结论:
- 诺斯胺不是由 Bacillus subtilis 合成的,并且在生理上与其生物膜形成无关.
- 上一项关于诺斯珀米丁在B. subtilis生物膜中的作用的研究结果与之前的研究结果相矛盾.
- 外源的诺斯珀米丁可以抑制B. subtilis的生长和生物膜的形成,这种方式与外聚糖类无关.
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