植物双亲关系酸盐输送器的晶体结构 NRT1.11.1
Ji Sun1, John R Bankston2, Jian Payandeh3
1Department of Pharmacology, Box 357280, University of Washington, Seattle, Washington 98195, USA.
Nature
|February 28, 2014
概括
阿拉比多opsis NRT1.1 酸盐转运器作为二聚体,在低亲和度和高亲和度之间切换. 在Thr101的酸化控制了这种二分化,使双模式酸盐的吸收对植物生长至关重要.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 酸盐对于植物生长至关重要,但土壤中的度差异很大.
- 阿拉比多普西斯NRT1.1是一种双亲缘酸盐输送体,但其机制尚不清楚.
- 翻译后的修改,特别是Thr101的酸化,调节了NRT1.1的活动.
研究的目的:
- 阐明NRT1.1双亲关系传输的结构和分子机制.
- 了解 Thr101 酸化如何控制输送器活性和亲和状态.
- 为了研究二元化在NRT1.1函数中的作用.
主要方法:
- 进行X射线晶体学以确定未化NRT的结构1.1.1.
- 基于细胞的光共振能量转移 (FRET) 试验,用于研究膜中的二分化.
- 位点导向的突变发生 (Thr101的相仿性突变).
- 基板运输道的分析.
主要成果:
- 晶体结构显示非化NRT1.1形成一个向内面的同位素.
- Thr101酸化部位位于二元接口附近,将酸化与寡合状态联系起来.
- 功能性NRT1.1在细胞膜中进行二元化.
- 在Thr101的相仿性突变中断了二分化,从而产生了高亲和力传送器.
结论:
- NRT1.1使用受酸化控制的二分化开关进行双亲缘酸盐吸收.
- 这种机制允许植物在广泛的土壤度范围内适应酸盐的吸收.
- 对NRT1.1的结构洞察力为营养物质输送器调节提供了分子基础.
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