核毛孔保护基因组完整性,通过组装一个前基因和Mad1-依赖的酶抑制剂
Veronica Rodriguez-Bravo1, John Maciejowski1, Jennifer Corona1
1Molecular Biology Program and Louis V. Gerstner, Jr. Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
Cell
|March 4, 2014
概括
核孔复合体 (NPC) 和动态都会发出"等待相"信号. 这种双重信号通路确保了适当的染色体对齐,并在细胞分裂期间保持基因组完整性.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 螺旋组合检查点 (SAC) 对于通过延迟异位相,直到染色体正确对齐,来防止异位积分至关重要.
- 现有的模型表明,未连接的基内托霍尔通过产生酶促进复合物/循环体 (APC/C) 的抑制剂来产生SAC信号.
研究的目的:
- 研究核孔复合体 (NPC) 在调节轴组合检查点 (SAC) 中的作用.
- 为了确定NPC是否有助于酶抑制信号传递.
主要方法:
- 使用的Mad1-Mad2复合体与核孔复合体 (NPC) 结合.
- 评估了从NPC中取代Mad1-Mad2对相发作和错误纠正的影响.
- 采用异质的Mad1-NPC连接系统来恢复信号传输.
主要成果:
- 核孔复合体 (NPCs) 通过Mad1-Mad2复合体充当通过酶抑制信号传递的支架.
- 与NPC结合的Mad1-Mad2的干扰加速了阿纳相发作和损害了 merotelic 错误的纠正.
- 恢复Mad1-NPC连接正常化了"等待阿纳相"信号的产生和线粒控制.
结论:
- 核孔和动态都会发出"等待阿纳相"信号,建立一个冗余的系统来维持基因组完整性.
- NPC在SAC信号传递中起到以前未知的作用,有助于精确的染色体分离.
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