在kinetoplastids中发现了非常规的kinetochores
1Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK.
Cell
|March 4, 2014
概括
包括Trypanosoma brucei在内的kinetoplastids用于染色体分离的19种kinetochore蛋白 (KKT1-19) 的独特组合,挑战了真核生物中kinetochore组成的通用模型.
科学领域:
- 细胞生物学 细胞生物学
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- 基内托科尔是一个关键的蛋白质复合体,用于精确的染色体分离在真核细胞分裂.
- 在细胞生物学中,一个长期存在的假设是所有真核细胞体内的核心基内托科尔蛋白成分的普遍性.
- 作为一种独特的真核生物群体的基内托塑类动物缺乏已识别的常规基内托基因蛋白质,这就质疑了这种普遍性.
研究的目的:
- 为了识别和描述Trypanosoma brucei的kinetoplastid中的kinetochore蛋白质.
- 研究这些已识别的蛋白质在kinetoplastids中的进化保护和同质性.
- 挑战现有的普遍动态组合范式,并探索替代机制.
主要方法:
- 对Trypanosoma brucei基因组进行生物信息分析,以确定潜在的kinetochore蛋白质.
- 比较基因组学,以评估相关的kinetoplastid物种中识别的蛋白质的保存.
- 与已知的真核动态基因组数据库对抗蛋白质同类学搜索.
主要成果:
- 在Trypanosoma brucei.中鉴定了19种新的kinetochore蛋白,被指定为KKT1-19.
- 这些KKT蛋白质中的大多数在kinetoplastids中保存.
- 鉴定到的KKT蛋白质中,没有一种与已知来自其他真核生物的常规基内托科尔蛋白质具有同质性.
结论:
- 基内托塑类动物使用一组独特的蛋白质组装功能性基内托基因,与传统的真核生物模型有所不同.
- 这一发现提供了关键的证据,证明了基因细胞体染色体分离机制中独特的进化途径.
- 这些发现对了解真核生物的早期进化和真核树根中的kinetoplastids的位置具有重要意义.
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